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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Van Slyke determination ： ウィキペディア英語版 Van Slyke determination The Van Slyke determination is a chemical test for the determination of amino acids containing a primary amine group named after die american biochemist Donald Dexter van Slyke (1883-1971). Van Slyke was born in Pike (NY) and started his academic career at Rockefeller University in New York after earning his Bachelor's degree in Chemistry and a PhD in Organic Chemistry from the University of Michigan. One of his first achievements was the quantification of amino acids by the Van Slyke determination reaction. To quantify aliphatic amino acids, the sample is diluted in glycerol and then treated with a solution of sodium nitrite, water and acetic acid. The resulting diazotisation reaction produces nitrogen gas which can be observed qualitatively or measured quantitatively. Van Slyke Reaction: R-NH2 + HONO → ROH + N2 + H2O In addition, Van Slyke developed the so-called Van Slyke apparatus, which can be used to determine the concentration of respiratory gases in the blood, especially the concentration of sodium bicarbonate. This was of high importance to be able to recognize a beginning acidosis in diabetic patients as early as possible, in order to start alkali treatment. The Van Slyke apparatus became a standard equipment in clinical laboratories around the world and the results of Van Slyke's research are still used today to determine abnormalities in the acid-base homeostasis. Later on, Van Slyke further improved his apparatus, increasing its accuracy and sensitivity. Using the new method, he was able to further investigate the role of gas and electrolyte equilibria in the blood and how they change in response to respiration. ==References== 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Van Slyke determination」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.